General Cell Based Assays
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-free, 10 plates
The FLIPR Calcium 6-QF Assay Kit (Explorer kit) is a quench-free, mix-and-read fluorescent assay for measuring intracellular calcium flux in high-throughput plate formats. The Calcium 6-QF formulation provides an increased signal window and is suitable for quench-sensitive targets and multiplexed assays.
- Quench-free formulation for quench-sensitive targets and multiplexing.
- Designed for mix-and-read workflows with pre-optimized FLIPR and FlexStation protocols.
- Sufficient reagents for 10 plates in Explorer kit format.
- Each reagent vial for Sample and Explorer kits is sufficient for one 96-, 384-, or 1536-well plate.
- Suitable for endogenous, primary, and stem cell targets with improved signal window and assay robustness.
Molecular Devices Calcium Assay Kit, QF, Express, 96/384/1536-well
The FLIPR Calcium 6-QF Express Kit from Molecular Devices is a fluorescence-based, homogeneous calcium indicator assay designed for high-throughput intracellular calcium flux screening. The QF formulation supports quench-sensitive assays and multiplexed workflows, and the Express Kit supplies reagent vials sufficient for multiple microplates in 96-, 384-, and 1536-well formats.
- Homogeneous fluorescence-based calcium flux assay for cell-based screening.
- QF formulation for quench-sensitive targets and multiplexing.
- Contains 2 vials of Component A and 2 vials of Component C.
- Assay buffer (Component B) is not included in the Express Kit.
- Sufficient reagent for up to 100 96- or 384-well plates, or 150 1536-well plates.
- Suited for GPCR, ion channel, and other calcium signaling assays in high-throughput screening.
Molecular Devices Neurotransmitter Uptake Assay Kit, Fluorescence, Live-Cell, 96/384, 50-Plate
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, fluorescence-based live-cell assay for measuring uptake activity of serotonin (SERT), norepinephrine (NET), and dopamine (DAT) transporters. The kit uses a fluorescent substrate and masking dye to enable no-wash, mix-and-read endpoint or kinetic measurements on bottom-read microplate readers.
- Homogeneous, no-wash live-cell assay format.
- Fluorescence-based detection suitable for kinetic or endpoint reads.
- Compatible with 96-well and 384-well black clear-bottom plates.
- Bulk kit contains reagents for 50 plates (5 vials of lyophilized dye/masking mix).
- Reconstituted dye solution stable for limited time; store frozen reagents at -20°C.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 10pl Kit. (93-0719E3CP5L)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 10pl Kit. (93-0729E3CP0L)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRM1 Total GPCR Internalization Assay, 10pl Kit. (93-0745E3CP5L)
The PathHunter OPRM1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRM1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged OPRM1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRM1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 Activated GPCR Internalization Assay, 2pl Kit. (93-0710E3CP0M)
The PathHunter AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical GlycolysIs Cel-Based asy K 1ea
Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.
Eurofins Discoverx PathHunter CNR1 Bioassay Kit, 10-Plate. (93-0959Y2-00120)
The PathHunter CNR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CNR1 and EA-tagged β-Arrestin-2. Activation of CNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress ErbB2/ErbB4 Dimerization Assay, 2pl Kit. (93-0960E3CP5M)
The PathHunter ErbB2/ErbB4 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. heregulin β-1) induced dimerization of ErbB2/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB2/ErbB4, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR120S (Short Isoform) CHO-K1 β-Arrestin GPCR Assay, 2-plate Kit. (93-0924E2CP0M)
The PathHunter GPR120S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of GPR120S GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR120S and EA-tagged β-Arrestin-2. Activation of GPR120S stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.