General Cell Based Assays
Immunology Consultants Hamster (CHO) Cathepsin B - Recombinant, 0.025mg
Recombinant CHO Cathepsin B (CTSB; UniProt G3H0L9) is expressed in CHO cells with a 6xHIS tag and purified to >95% purity. The protein appears at ~45 kDa under reducing conditions and is quantified by A280. Cathepsin B is a dual‑function cysteine protease with both endopeptidase and carboxypeptidase activity, making it a significant CHO‑derived impurity capable of fragmenting therapeutic proteins. Its presence has been linked to antibody degradation in bioprocessing. This high‑quality recombinant antigen provides a reliable standard for ELISA development, antibody generation, and impurity profiling. Its CHO‑derived structure ensures biological relevance for protease‑risk evaluation and purification optimization. A strong choice for teams developing targeted HCP detection tools or assessing protease‑driven degradation.
Sigma Aldrich Fine Chemicals Biosciences D-2-HYDROXYGLUTARATE (D2HG) KT
D-2-Hydroxyglutarate (D2HG) Assay Kit
Revvity Health Sciences Inc HTRF cAMP Gs Dynamic & Gi Detection Kit Standard
HTRF cAMP Gs Dynamic & Gi Detection Kit Standard
Eurofins Discoverx PathHunter Human SREBP2 Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human SREBP2 Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure cholesterol biosynthesis pathway activation via nuclear translocation of SREBP2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress OSMRb/IL31RA Dimerization Assay, 10pl Kit. (93-1002E3CP5L)
The PathHunter OSMRb/IL31RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31RA receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NItrateNItrIte ColorImetrIc
Cayman’s Nitrate/Nitrite Colorimetric Assay Kit provides an accurate and convenient method for measurement of total and individual nitrate and nitrite concentrations in a simple two-step process utilizing nitrate and nitrite standards. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step is the addition of the Griess Reagents which convert nitrite into a deep purple azo compound (See Figure 2).{1191} Photometric measurement of the absorbance due to this azo chromophore accurately determines NO2- concentration. This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be used to analyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH has been added. For these assays a second method (LDH method) is utilized and is available from Cayman Chemical in a 96 well plate format (Item No. 760871).
Eurofins Discoverx cAMP Hunter eXpress NPY2R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0077E2CP2L)
The cAMP Hunter NPY2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPY2R, a naturally Gi/Go coupled GPCR. Upon activation of NPY2R, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Green)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CXCR6 HEK 293 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0205E1CP0L)
The PathHunter CXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of CXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR6 and EA-tagged β-Arrestin-2. Activation of CXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc ETV4 Transcription Factor Activity Assay
ETV4 Transcription Factor Activity Assay
Eurofins Discoverx PathHunter eXpress GPR85 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0409E2ACP1L)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices Fatty Acid Uptake Assay, Homogeneous Fluorescence, 96/384-well
The QBT Fatty Acid Uptake Assay Kit is a one-step, homogeneous fluorescence assay for monitoring cellular uptake of fatty acids and screening fatty acid transport activity in live cells. It enables real-time kinetic measurements in 96- and 384-well plate formats and is optimized for high-throughput workflows.
- One-step homogeneous assay format for streamlined workflow.
- Uses a BODIPY-dodecanoic acid fluorescent fatty acid analog for physiological relevance.
- Proprietary quench dye minimizes extracellular signal for improved signal-to-background.
- Compatible with 96-well and 384-well plates for high-throughput screening.
- Pre-optimized reagents supplied to reduce assay development time.
- Eliminates need for radioactive tracers, reducing disposal and safety concerns.